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Development of culture media for cell mass and secondary metabolites production of Bacillus sp. for controlling bacterial leaf blight disease of rice
Thesis Abstract:
The high cost in laboratory culture media is a major challenge for microbiological studies especially in developing countries. Legumes are well-known crop with high carbon and nitrogen contents which support the growth of bacteria including vitamins and minerals. The study was carried out to find the cost-effective media from locally available legumes (black bean, mung bean, and soybean) for the cell multiplication and secondary metabolites production of Bacillus amyloliquefaciens S20A1, the beneficial antagonistic bacteria, to control bacterial leaf blight disease of rice caused by Xanthomonas oryzae pv. oryzae. Maximum cell growth and secondary metabolites production were observed at two modified media formula, black bean + soybean mixture medium (BS) and mung bean + soybean mixture medium (MS) with 1.00 x 10 x 10 CFU /ml and 1.3 x 10 (to 10th power) CFU /ml respectively. Additionally, these two modified media showed the higher potential than the general medium, Luria Bertani broth. The modified media composition of 30 grams per liter (1:1 ratio) incubated on 150 rpm rotary shaker for 36 hours were the most effective culture conditions. Although three types of powder carrier, talcum, kaolin and talcum + kaolin mixture, showed not significantly different effects on bacterial survival for 90 days storage, talcum + kaolin mixture formulation examined the highest disease reduction 68.33% and 67.40% when applied with BS and MS modified media respectively. The two bean extract modified media can be used as a cost-effective media and could be used to provide a reliable basis for a scale-up to an industrial level.
