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Alternative Low-cost, Non-mist Macropropagation Technology for Mamalis [Pittosporum pentandrum (Blanco) Merr.] as Affected by the Location of Cuttings and Different Concentrations of Indole-3-Butyric Acid
Dissertation Abstract:
A greenhouse nursery experiment was conducted at the Tarlac College of Agriculture in Camiling Tarlac to determine the best location of cuttings in the ortet and the appropriate level of concentration of Indole-3-butyric acid for the macropropagation of mamalis under low-cost mist propagator.
Uniform two-node cuttings were collected from the lower and upper sections in the ortet of a 1 to 1/2-year-old leafy stem cuttings of mamalis. The stem cuttings were planted in hiko trays filled with coconut coir dust and ordinary fine river sand mixed at a ratio of 2:1. The cuttings were treated with various concentrations of indole-3- butyric acid (IBA) from 0 ppm (control), and 2000, 3000, 4000, 5000, and 6000 ppm. These were placed and tended inside a fabricated mistless propagation chambers in the greenhouse for 90 days from 17 July to 15 October 1997. Root production, shoot development, and percentage survival were assessed.
The location of cuttings in the ortet did not differ significantly in terms of number of roots, length of roots, percent callused, root dry weight, number of shoots, and percentage survival per cutting. Similarly, the combined effects of the location of cuttings in the ortet and the different concentration of indole-3-butyric acid did not vary significantly in terms of root production, shoot development, and percentage of survival. Cuttings of mamalis taken from a juvenile stock plant showed that the species can be propagated under low-cost mistless propagation chambers.
On the other hand, irrespective of the location of the cuttings in the ortet, the different concentrations of IBA significantly produced more roots, greater number of shoots, longer roots with higher root dry weight, and percentage callusing per cutting. The treatment of IBA at different levels of concentration promoted or enhanced root production, shoot development, and percentage survival.
The stem cuttings treated with 4000 and 5000 ppm IBA consistently registered the best performance in terms of root production, shoot development. They also recorded the highest percentage of survival per cutting and the least was 0 ppm (control). Most of the cuttings rooted after three months.
Generally, there is a great potential of using low-cost, non-mist propagators using juvenile plant material and appropriate level of concentration of IBA in the mass production of quality planting stock of mamalis.