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Sulfide Precipitation of Mercury, Lead and Cadmium using Sulfate Reducing Bacteria Isolated from Cisolok and Muara Angke
Thesis Abstract:
The use of microorganism to control environmental pollution problem due to heavy metals had been increasing. At certain condition, sulfate reducing bacteria (SRB) could reduce sulfate compound to sulfide and are potential to control sulfate and heavy metals.
This research aimed to isolate sulfate reducing bacteria group from Muara Angke, Jakarta as well as from Cisolok hot spring water, Sukabami; and to obtain information on the effect of temperature and incubation period on the sulfide formation of mercury (Hg), lead (Pb) and cadmium (Cd) using hydrogen sulfide (H2S) produced by SRB group.
Each of the five isolates of SRB group had been isolated from the two mentioned locations of which superior isolate was selected to determine the effect of temperature (35, 50 and 65°C) and incubation period (7, 14 and 21 days) on the sulfide formation of the metals Hg, Pb and Cd as well as sulfate concentrations.
In this research, five each of the SRB superior isolates from Muara Angke and Cisolok were obtained (in the form of mixed culture), which could reduce sulfate and precipitate sulfide of the metals Hg, Pb and Cd. The highest reduction percentage of sulfate was obtained from Cisolok isolate (37.88%); and the reduction percentage of metal solution to its sulfide precipitation: Hg (55.53%), Pb (50.12%) and Cd (36.62%).
The highest percentage of sulfide metal compound formation of SRB isolate from Cisolok and Muara Angke was obtained at 21 days of incubation. Compared with SRB isolate from Muara Angke, the Cisolok isolate could reduce more sulfate to its sulfide and precipitate sulfide metal compound of Hg, Pb, and Cd higher than Muara Angke isolate.
