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Morpho-anatomical Development, Nitrogen and Starch Contents, and Auxin Levels Druing Graft Healing in Pili (Canarium Ovatum Engl.)
Dissertation Abstract:
The morpho-anatomical development during graft healing and union in pili (Canarium ovatum Engl.) was investigated. Four major events were observed weekly for four weeks in patch budding and monthly for four months in inarching. Apparent unioin was attained at Event 3, wherein differentiation of the cambiform arcs or cambial connections occurred.
Graft healing and unioin in pili seemed to belong to the category of cell differentiation involving cambial connections. It was not known whether differentiation of callus mass in the wood area to new vascular elements in inarching occurring very late in the development. Time constraint was a limitation. Stem growth was apparent in inarching as revealed by the constriction developed at union secured by the plastic strip.
Chemical components of the leaf, stem, and root of the inarched plants were analyzed following the developmental events. Significant decrease and increase in the chemical components (nitrogen and starch contents) of the organs sustained the processes of graft healing and union. The decrease in the chemical components in the leaf and root might not be due to degradation and translocation to the stem. The increase in the chemical components of the organs seemed to show synthesis which occurred when graft union was attained for successfully inarched plants.
A similar trend was shown for total auxin-like activity but the decrease and increase were insignificant. High correlation was revealed by the chemical components of the scion and stock at grafting time with percent success.
The reported favorable response of pili to grafting using dormant scions was explained, clarified, and partially supported under specific conditions. Strain F14 responded favorably in preference to inarching. Variations due to apparent strain adaptability were recognized for F9 and F18. The overall percent success was low for the two scion stages. The low percent success obtained was qualified through interaction effects of the strain used and method of propagation.
