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Genetic Diversity Based on Phenotype and SSR Markers and Identification of SNP in FSHR Gene Associated with Egg Production Traits in Philippines Mallard Duck (Anas platyrhynchos domesticus L)
Dissertation Abstract:
A total of 559 adult Philippine mallard ducks were sampled from Davao del Norte, Iloilo, Pampanga, and Quezon for phenotypic characterization. Out of these, 120 were used for a simple sequence repeat (SSR) analysis. Ducks in the four provinces were morphologically diverse and exhibited moderate to high genetic differentiation with ducks from Davao del Norte and Quezon having the farthest distance. The phylogenetic analysis based on SSR showed that ducks from Davao del Norte and Iloilo grouped into one cluster, while ducks from Pampanga and Quezon grouped into another. A total of 175 female Pateros and Itik Pinas Itim mallard ducks were raised since day-old up to 40 weeks of age to identify the single nucleotide polymorphisms (SNP) in the follicle-stimulating hormone receptor (FSHR) gene associated with egg production. Itik Pinas Itim line had a more uniform appearance, was significantly larger, and laid eggs one week earlier than the Pateros type. The Exon 10 region of FSHR gene spanning 640 bp was amplified and was found to be highly conserved among the Anatidae family. Two nucleotide variants (p1664C>A and p1988G>A) were detected within the Pateros population but based on rare allele frequency, only the p1664C>A can be considered as putative SNP. No consistent trend was observed for egg production traits. This is the first study to identify polymorphism in FSHR gene of Philippine mallard duck.