Skip to main content

Search Filter

Keywords:

 

Asian Journal of Agriculture and Development (AJAD) - Call for papers!

The Occurrence of ESBL-Producing E. coli in Chicken Slaughterhouses of Pondok Rumput Kota Bogor

(Indonesia), Master of Science in Veterinary Public Health (Institut Pertanian Bogor)

Thesis Abstract:

Chicken slaughterhouses play an important role in the spread of ESBL-producing E. coli in the meat supply chain. The aim of the study was to detect the occurrence of ESBL-producing E. coli in chicken slaughterhouses in Pondok Rumput Kota Bogor. ESBL-producing E. coli isolates (n = 20) were collected from 84 samples and were identified using API 20E kit. Samples were collected from seven different locations for a period of one week. ESBL in this study was confirmed using MAST D68C kit. A total of 20 ESBL-producing E. coli isolates were tested against 18 antimicrobial agents using the disc diffusion method and the interpretation of resistance was conducted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI). ESBL-producing E. coli isolates showed resistance against all of 18 antimicrobial agents and 100 percent of the isolates showed resistance against more than 9 antibiotics (multidrug resistance). The isolates were resistant to penicillin (100%), amoxicillin (100%), ampicillin (100%), cefotaxim (100%), cefpodozime (100%), ceftazidim (100%), streptomycin (95%), gentamycin (85%), trimetoprim-sulphamethoxazole (85%), tetracycline (70%), kanamycin (65%), ciprofloxacine (60%), norfloxacine (55%), doxycycline (45%), colistin sulfate (45%), cephalothin (30%), neomycin (15%), and polymyxin B (15%).

 

ESBL-producing E. coli bacteria derived from chicken slaughterhouses (n = 20) were found on the carcass containers (20%), offal containers (10%), floor of carcass and offal handling area (25%), knives (15%), water tank (5%) and feather puller machine (25%). The existence of resistant bacteria in chicken slaughterhouses could lead to transmission of resistance genes to other bacteria that could spread to the carcass, which could be potentially harmful to humans.