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Cortisol Hormones and Blood Biochemical Profile of Repeated Breeding Indonesian Local Cattle
Thesis Abstract:
This study was conducted to determine cortisol hormone and blood biochemical profiles of Indonesian local cattle with repeated breeding. Thirty cattle of three breeds udes in this study were Bali cattle in Bali Island, Madura cattle in Madura Island and Ongole crossbreeds in Yogyakarta. Each breed of cattle consisted of 2 groups, each of which consisted of 5 fertile cows and 5 cows with repeat breeding. Approximately, 3 ml blood samples were taken from jugular vein, and analyzed using a blood biochemical analysis, cortisol hormone analysis, and a feed proximate analysis. The variables observed were cortisol and blood biochemical profiles (phosphorus, total protein, and blood urea nitrogen). Cortisol hormone measurement was performed by ELISA using a commercial cortisol hormone kits (DRG, Germany), while a biochemical measurement was performed by a spectrophotometer method using a Microlab 300 photometer. The collected data were analyzed by two-ways ANOVA, followed by Duncan's New Multiple Range Test (DMRT) if the results showed a significant effect. The statistical tests of dry matter intake, organic matter, crude fiber, crude protein, crude fat, and total digestible nutrient (TDN) in the three cattle breeds showed significant difference (P< 0.05) and the highest was found in Ongole crossbreed compared to Madura and Bali cattle. Furthermore, statistical tests on dry matter intake, organic matter, crude fiber, crude protein, crude fat, and total digestible nutrient in fertile cows and those with repeat breeding did not show a significant difference. The results show that the highest average of the cortisol hormone profile in Bali cattle was 62.48 ± 32.77 mg/dl followed by Ongole Crossbreed 28.11 ± 13,55 mg/dl and the lowest was in Madura cattle 17.63 ± 5.41 mg/dl. The highest profile of cortisol hormone was found among cattle with repeated breeding compared to fertile cows 44,17 ± 4,62 vs. 27.97 ± 4.62 mg/dl). The results of blood biochemical analysis showed that the highest blood phosphorus and total protein was found in Madura cattle (6.45 ±1.50 and 8.08 ± 0.44 mg/dl) while the highest profile of blood urea nitrogen was found in Bali cattle (42.54 ± 16.61 mg/dl). The highest blood phosphorus and total protein were found in fertile cows (5.99 ± 0.39 and 7.40+0.26 mg/dl), while the highest blood urea nitrogen was found in cattle with repeat breeding (33.31 ± 3.23 mg/dl). It can be concluded that nutrient consumption of feed on the three breeds of cattle is lowest in Bali cattle, but the profile of cortisol hormone and blood nitrogen urea was the highest if compared to Madura cattle and Ongole crossbreds. The intake of nutrient in cattle with repeated breeding was low, but the cortisol hormones and blood urea nitrogen were the highest if compared to those of fertile cows.