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Potential Improvement of Unused Tiger Prawn (Penaeus monodon Fab.) by Hormonal Treatments
Abstract:
Unused tiger prawn (Penaeus monodon Fab.) is one that has undergone ablation after only two breeding periods, which causes high production cost, pollution of the environment, and difficulties in providing it continuously with enough quantity at exact time. The objectives of this study were to (1) find the proper dosage of dopamine, ecdyson, prostaglandin F-2α, and estradiol treatments, which will give the best quality for breeding of the unused tiger prawn; (2) determine the presence of prostaglandin and estradiol receptors histochemically; and (3) identify prostaglandin, and estradiol profile in the maturation of female prawn by immunohistochemical methods.
The first stage of this research involved collection of samples. All of the samples were twice breeding tiger prawn taken from the same sources (Aceh). The samples were treated with different dosage of ecdyson, prostaglandin, dopamine, and estradiol. The parameters identified in this experiment were optimal dosage, reproduction ability, fecundity, hatching rate, and egg quality.
The second stage was concerned with larvae rearing, identifying the parameters of growth, survival rate, and larvae quality, i.e., feeding activities, movement activities, behavior, coloring, and body and organ development.
The third stage was identifying the existence of prostaglandin and estradiol receptors histochemically (gold colloid), and hormone profile by immunohistochemical methods.
The results of this research are expected to give contribution to science with the information on prostaglandin and estradiol receptors in prawn, and optimum dosage to improve vitellogenesis, egg and larva quality. It will also support the improvement on the prawn industries by low cost production since the prawn will be reused after breeding twice, and will protect the loss of tiger prawn from the Indonesian marine territory.