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Production and Utilization of Cellulase Enzymes from Trichoderma viride to Improve Rice Straw Silage Quality
Abstract:
This research was conducted in two stages: (1) production and activity test of crude enzymes from Trichoderma viride, and (2) comparison test using crude enzymes from T. viride strain QM 9414 (CETV) and cellulose commercial ezyme (cellulose “Onozuka R-10,” Heidelberg) on rice straw silage. The treatments were arranged as follows: P0 = untreated rice straw silage, P1 = treated by cellulase commercial enzyme at 10 IU kg-1, P2 = treated by CETV at 3.8 IU kg_1, P3 = treated by CETV at 7.6 IU kg-1, and P4 = treated by CETV at 11.4 IU kg-1 of fresh rice straw. All treatments were enriched with 5 percent molases.
The results indicated the following: (1) the activity of crude enzymes from T. viride strain (QM 9414 (CETV) was 1.52 IU ml-1; (2) the addition of both enzymes did not decrease dry matter of rice straw silage, however, organic matter significantly decreased by the addition of cellulase commercial enzyme, (3) the addition of both enzymes decreased fiber contents (acid detergent fiber and neutral detergent fiber) and increased in sacco digestibility of dry matter and organic matter of rice straw silage.