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Asian Journal of Agriculture and Development (AJAD) - Call for papers!

Comparative evaluation of various methods for the detection of Listeria monocytogenes (Murray et al.) in selected raw and processed meat products

(Indonesia), Master of Science in Food Science (University of the Philippines Los Baños)

Thesis Abstract:

The study was conducted to compare selected methods that detect the presence of Listeria monocytogenes in selected raw and processed meat products. Results indicated that rapid methods (PCR-based and VIDAS-LDUO®) detected L. monocytogenes faster than the conventional method. PCR-based method was fastest, followed by VIDAS-LDUO®, and then the conventional method. It was also gathered that the VIDAS-LDUO® method was the least sensitive as it occasionally failed to detect L. monocytogenes even in spiked samples.
 
API Listeria kit was also used to determine the phenotypic characteristics of isolated Listeria species. Two strains of L. monocytogenes isolated from meat products possessed unique characteristics as determined by 16S rRNA gene sequencing. They were previously undocumented at the Philippine National Collection of Microorganisms (PNCM) and were therefore added to their L. monocytogenes strain collection.