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Effects of Type of Inocula and Phosphate Levels on Mycorrhizal Formation and Initial Growth of Pinus Merkusii Jungh et de Vriese Seedlings in Two Types of Soils
Thesis Abstract:
The study determined the effects of soiI type, superphosphate levels, inocula types as well as the combined effects of soil and superphosphate level, soil and inoculation type, and soil superphosphate level and inoculum type that favor the initial growth of Pinus merkusii.
Seeds were obtained from Bandung, Indonesia. Soils were colleted from Caliraya, Kalayaan, and the Forest Experimental Planation, College of Forestry, University of the Philippines Los Baños (UPLB-CF), both in Laguna. Seedlings were inoculated with spores of fungi tentatively identified as Pisolithus tinctorius and Rhizopogon sp. or mycorrhizal pine soil one month after germination and the fertilized with superphosphate two months later.
Soil type significantly affected diameter growth, root/shoot ratio, and mycorrhizal root formation. Growth of P. merkusii seedlings was better in Caliraya soil than in UPLB-CF soil.
Phosphate levels did not affect growth parameters.
Inoculum type significantly affected height, diameter, total biomass, root/shoot (R/S) ratio, and mycorrhizal root formation. Uninoculated seedlings showed significantly greater growth than all inoculated seedlings, but in terms of mycorrhizal formation, mycorrhizal pine soil was the best.
Combination of soil type and superphosphate level significantly affected diameter growth. Seedlings planted in Caliraya soil fertilized with four, six, and eight superphosphate per seedling had greater diameter growth than those planted in UPLC-CF soil fertilized with any level of superphosphate. Combination of soil type and inoculum type significantly affected total biomass, R/S ratio, number of soil types, superphosphate levels, and inoculum type did not affect any of the growth parameters.
It was concluded that poor soil like Caliraya soil induced better diameter growth, R/S ratio, and mycorrhizal root formation of P. merkusii than fertile soil like UPLB-CF soil. Results showed that the superphosphate levels used in the experiment did not affect growth of P. merkusii seedlings and that mycorrihizal pine soil was a better inoculum that the pure culture inoculum of Pisolithus tinctorius and Rhizopogon sp.