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The Effects of Tree Age, Light Quality, Stratification, and Chemical Treatments on Seed Germination and Seedling Growth of Pinus Merkusii
Thesis Abstract:
Pinus merkusii seeds collected from parent trees of various ages (11-15, 16-20, 21-25, and 26-30 years) were exposed to varying light quality, stratification, KNO3,GNO3, H2O2 treatments. Seed germination and seedling growth were observed.
Seed germination was significantly higher in seeds collected from parent trees 11-15 and 16-20 years old as compared to seeds collected from the other parent trees. Similarly, seedlings derived from seeds collected from parent trees 11-15 and 16-20 years old gave the best seedling growth in terms of dry weight, height, and diameter.
Seed germination was significantly affected by light quality treatments. Normal and red light treatments gave higher seed germination than blue, far-red, and dark treatments. Correspondingly, seedlings exposed to normal and red light exhibited the best growth in terms of dry weight, height, and diameter.
Seed germination and seedling growth were not significantly affected by KNO3 treatments. However, treatment with GA3 significantly improved germination. The highest germination was obtained by seeds treated with 500 pm GA3. Seedling growth was not affeced by GA3 treatments. H2O2 treatments was significantly improved by seeds treated with 30 percent H2O2 in terms of shoot/root ratio and dry weight.
Stratification treatment significantly improved seed germination. Highest germination was obtained by seeds placed in moist, steralized vermiculite in the refrigerator for about two weeks. However, stratification treatments did not significantly affect seedling growth.
Finally, there was improvement in germination of seeds colleced from parent trees 11-15 and 16-20 years old, exposed to normal and red light, treated with 500 ppm GA3, and placed in moist, sterilized vermiculite for about two weeks. Similarily, P. merkusii, seedlings which were from seeds collected from parent trees 11-15 and 16-20 years old, exposed to normal light and red light, and treated with 30 percent H2O2 had grown the most.